In Vitro Labeling of Rna in Isolated Nucleoli of the Walker Tumor and Liver.

نویسندگان

  • T S RO
  • H BUSCH
چکیده

ethanol was also required for the optimal activity. The pH optimum was 8.2 for both tumor and liver nucleoli. Maximum incorporation of isotope into RNA was found at 15-30 minutes after the incubation was begun. The reactions were DNA-dependent, as shown by sensitivity to actinomycin D. Evidence for internucleotide incorporation of ATP-P32 was obtained by analysis of P32distribution into nucleotide monophos-

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In Vitro Labeling of RNA in Isolated Nucleoli of the Walker Tumor and Liver* TAB SUK Ro AND HARRIS BUSCH

ethanol was also required for the optimal activity. The pH optimum was 8.2 for both tumor and liver nucleoli. Maximum incorporation of isotope into RNA was found at 15-30 minutes after the incubation was begun. The reactions were DNA-dependent, as shown by sensitivity to actinomycin D. Evidence for internucleotide incorporation of ATP-P32 was obtained by analysis of P32distribution into nucleot...

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عنوان ژورنال:
  • Cancer research

دوره 24  شماره 

صفحات  -

تاریخ انتشار 1964